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Killing of Bacillus Spores by High-Intensity Ultraviolet Light

STUDY ON EFFECTS OF PULSED LIGHT

Rationale
 
Bacterial endospores, of the type by Bacillus and Clostridium species, are known to be highly resistant to various forms of radiation and other physical and chemical agents. High-intensity ultraviolet light, however, was suspected to overcome such resistance and to kill spores efficiently. To test this hypothesis, I irradiated samples of Bacillus subtilis spores (in collaboration with Roger Williams, Xenon Corporation) and measured the loss of viability as a function of UV dose and position with respect to the axis of the lamp.
 
Protocol

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